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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A laboratory worker sustains a needlestick injury while handling a blood sample known to be positive for Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg). The worker has completed the HBV vaccination series and has a documented anti-HBs titer >10 mIU/mL one month ago. What is the recommended post-exposure prophylaxis?

A) Hepatitis B immune globulin (HBIG) only
B) Hepatitis B vaccine booster dose only
C) Both HBIG and HBV vaccine booster dose
D) No treatment required

2. A clinical microbiology laboratory is evaluating a new multiplex PCR assay for the detection of respiratory viruses. To assess the assay's specificity, they test a panel of samples known to contain various bacterial pathogens. A positive result is obtained for rhinovirus in a sample containing only Streptococcus pneumoniae.
This indicates:

A) A mixed infection in the original patient sample.
B) Poor analytical sensitivity of the PCR assay for rhinovirus.
C) Degradation of the rhinovirus target sequence during storage.
D) Cross-reactivity of the PCR primers with Streptococcus pneumoniae DNA.

3. A patient presents with perianal itching, particularly at night. Microscopic examination of cellophane tape preparation from the perianal area reveals oval-shaped eggs with one flattened side. The MOST likely causative organism is:

A) Trichuris trichiura
B) Strongyloides stercoralis
C) Ascaris lumbricoides
D) Enterobius vermicularis

4. A 30-year-old female who recently returned from a hiking trip in the northeastern United States presents to her physician with a two-week history of a slowly expanding, erythematous rash with central clearing on her thigh. She also reports intermittent fatigue, arthralgias, and mild headache. Serological testing reveals a positive IgM and IgG response to Borrelia burgdorferi. Which of the following mechanisms is MOST significant in the pathogenesis of the clinical manifestations observed in this patient?

A) Production of potent exotoxins leading to direct tissue damage
B) Evasion of phagocytosis through a thick polysaccharide capsule
C) Intracellular replication within macrophages leading to disseminated infection
D) Induction of a strong pro-inflammatory cytokine response by the host

5. During a community outbreak of influenza, a public health laboratory performs rapid antigen testing on nasopharyngeal swabs. A test for influenza A is positive, but the influenza B test is negative. Subsequently, RT-PCR is performed on the same sample, and it is negative for both influenza A and B viral RNA. The MOST likely explanation for this discordant result is:

A) The patient was infected with a novel influenza strain not detected by either assay.
B) The rapid antigen test has low sensitivity, resulting in a false positive.
C) The rapid antigen test yielded a false positive result, and the RT-PCR was a true negative.
D) The RT-PCR assay has a high limit of detection, missing a low viral load.

Solutions: